This reports the protocol used to align the RiceAlta_BACend_OA_BB_OMAP-20070815 features to indica-genome. Tue Aug 21 12:05:28 2007 Source of RiceAlta_BACend_OA_BB_OMAP-20070815 : from Gramene markers database, originally Downloaded from genbank nucleotide databse with keyword 'OA_BBa' Alignment procedure details --------------------------- 128732 RiceAlta_BACend_OA_BB_OMAP-20070815 are aligned to indica-genome using blat with blat parameters -minIdentity=50 -minScore=10 -maxGap=3 followed by PslReps with -minNearTopSize=75 -minAli=0.25. This was followed by a filtering procedure described below and applied in general to 'OMAPSpecies-Genomic' data sets. Initial summary # alignments : 131694 # unique Features these alignments represent: 108075 % of total features these alignments represent : 83.95 % The length of the matches are distributed as follows Hit_length # alignments -------- -------- 100 13814 150 14608 200 10815 250 10602 300 10346 350 10332 400 10455 450 9913 500 9261 550 8469 600 7252 650 6270 700 4723 750 2885 800 1439 10000 510 gap distribution of the remaining features gaps # alignments -------- -------- 1000 117830 2000 868 3000 443 4000 322 5000 245 6000 261 7000 140 8000 124 9000 131 10000 91 20000 973 Frequency distribution of the remaining features # hits # features -------- -------- 1 93830 2 9808 3 2463 4 881 5 427 6 257 8 237 9 60 10 45 20 64 30 3 40 0 50 0 100 0 % Identity distribution of the remaining features % Identity # alignemnts -------- -------- 10 0 20 0 30 14 40 80 50 318 60 969 70 6469 80 13372 90 40449 100 70023 Following is the final summary # alignments : 131694 # unique Features these alignments represent: 108075 % of total features these alignments represent : 83.95 %
Last modified: Thu Sep 13 15:01:03 2007