This reports the protocol used to align the Maize_Seq_Panzea features to Oryza_sativa_indica-chromosome-20070724. Tue Aug 7 14:54:34 2007 Source of Maize_Seq_Panzea : Obtained from panzea project Alignment procedure details --------------------------- 3092 Maize_Seq_Panzea are aligned to Oryza_sativa_indica-chromosome-20070724 using blat with blat parameters -minIdentity=50 followed by PslReps with -singleHit. This was followed by a filtering procedure described below and applied in general to 'CrossSpecies-Coding' data sets. Initial summary # alignments : 144 # unique Features these alignments represent: 113 % of total features these alignments represent : 3.65 % The length of the matches are distributed as follows Hit_Length # alignments -------- -------- 100 88 150 18 200 11 250 8 300 6 350 2 400 1 450 2 500 2 550 1 600 1 650 1 700 1 750 2 800 0 10000 0 Alignments with matches less than 150 bp are deleted # remaining Alignments : 39 # unique Features these remaining alignments represent: 34 % of total features these alignments represent : 1.10 % Frequency distribution of the remaining features # hits # features -------- -------- 1 30 2 3 3 1 4 0 5 0 6 0 8 0 9 0 10 0 20 0 30 0 40 0 50 0 100 0 Features that hit more than thrice are deleted. # remaining Alignments : 39 # unique Features these remaining alignments represent: 34 % of total features these alignments represent : 1.10 % % Identity distribution of the remaining features % Identity # features -------- -------- 10 0 20 0 30 0 40 0 50 0 60 0 70 0 80 0 90 0 95 31 100 8 Following is the distribution of gaps Gaps # features -------- -------- 1000 34 2000 3 3000 0 4000 0 5000 1 6000 0 7000 0 8000 0 9000 0 10000 0 Following is the final summary # alignments : 39 # unique Features these alignments represent: 34 % of total features these alignments represent : 1.10 %
Last modified: Thu Sep 13 15:01:03 2007