Reference "Genetic dissection of a genomic region for a quantitative trait locus, Hd3, into two loci, Hd3a and Hd3b, controlling heading date in rice"

Reference ID

9518

Title

Genetic dissection of a genomic region for a quantitative trait locus, Hd3, into two loci, Hd3a and Hd3b, controlling heading date in rice

Source

Theor Appl Genet, 2002, vol. 104, pp. 772-778

Authors (5)

Monna-L	Lin-X
Kojima-S	Sasaki-T
Yano-M

Abstract

The rice photoperiod sensitivity gene Hd3 was originally detected as a heading
date-related quantitative trait locus localized on chromosome 6 of rice. High-
resolution linkage mapping of Hd3 was performed using a large segregating
population derived from advanced backcross progeny between a japonica variety,
Nipponbare, and an indica variety, Kasalath. To determine the genotype of Hd3,
we employed progeny testing under natural field and short-day conditions. As a
result, two tightly linked loci, Hd3a and Hd3b, were identified in the Hd3
region. Nearly-isogenic lines for Hd3a and Hd3b were selected from progeny using
marker-assisted selection. The inheritance mode of both Hd3a and Hd3b was found
to be additive. Analysis of daylength response in nearly-isogenic lines of Hd3a
and Hd3b showed that the Kasalath allele at Hd3a promotes heading under short-
day conditions while that at Hd3b causes late heading under long-day and natural
field conditions.