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E.g., Wessler, regeneration, PubMed ID 17578919.

expand all sections collapse all sections  Reference "Characterization and detection of epistatic interactions of 3 QTLs, Hd1, Hd2, and Hd3, controlling heading date in rice using nearly isogenic lines"
Reference ID 6999
Title Characterization and detection of epistatic interactions of 3 QTLs, Hd1, Hd2, and Hd3, controlling heading date in rice using nearly isogenic lines
Source Theoretical and applied genetics, 2000, vol. 101, pp. 1021-1028
Authors (4)
Abstract To characterize quantitative trait loci (QTLs), we used marker-assisted
selection (MAS) to develop three nearly isogenic lines (NILs) differing only for
the presence of a single, specific QTL (QTL-NILs) - Hd1, Hd2, and Hd3 - for
heading date in rice. The three lines contained the chromosomal region of the
target QTL from donor variety Kasalath (indica) in the genetic background of
var. Nipponbare (japonica). To analyze epistatic interactions in pairs of these
QTLs, we also used MAS to develop four combined QTL-NILs with 2 of the 3 QTLs or
with all 3. Different daylength treatment testing of the QTL-NILs revealed that
the three QTLs control photoperiod sensitivity. Genetic analysis of F2
populations derived from crosses between the three QTL-NILs with a single QTL
using molecular markers revealed the existence of epistatic interactions between
Hd1 and Hd2, and Hd2 and Hd3. These interactions were also confirmed by the
analysis of combined QTL-NILs under different daylength conditions. The
existence of an epistatic interaction between Hd1 and Hd3 was also clarified.
Based on these results, we suggest that the Kasalath allele of Hd3 does not
affect photoperiod sensitivity by itself but that it is involved in enhancement
of the expression of the Nipponbare alleles of Hd1 and Hd2.

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