Reference ID | 6986 | ||||
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Title | Expression of the plasma membrane H+ -ATPase gene in response to salt stress in a rice salt-tolerant mutant and its original variety. | ||||
Source | Theoretical and applied genetics, 1999, vol. 99, pp. 1006-1011 | ||||
Authors (4) |
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Abstract | Plasma membrane (PM) H(+)-ATPase plays an important role in the establishment and maintenance of ion homeostasis. To investigate its expression in the rice salt-tolerant mutant M-20 and the original variety 77-170 during salt stress, a cDNA fragment corresponding to the PM H(+)-ATPase gene was obtained by PCR from rice japonica variety 77-170 and designated as OSA3. Sequence analysis of OSA3 revealed its high homology with two other published PM H(+)-ATPase genes, OSA1 and OSA2, in rice. Southern-blot analysis detected a RFLP between M-20 and 77- 170, and one copy of the OSA3 gene was mapped to a position on rice chromosome 12 where a salt tolerance QTL was closely located. |
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