Reference "Hd6, a rice quantitative trait locus involved in photoperiod sensitivity, encodes the alpha subunit of protein kinase CK2"

Reference ID

6941

Title

Hd6, a rice quantitative trait locus involved in photoperiod sensitivity, encodes the alpha subunit of protein kinase CK2

Source

Proceedings of the National Academy of Sciences of the United States of America, 2001, vol. 98, pp. 7922-7927

Authors (4)

Takahashi-Y	Shomura-A
Sasaki-T	Yano-M

Abstract

Hd6 is a quantitative trait locus involved in rice photoperiod sensitivity. It
was detected in backcross progeny derived from a cross between the japonica
variety Nipponbare and the indica variety Kasalath. To isolate a gene at Hd6, we
used a large segregating population for the high-resolution and fine-scale
mapping of Hd6 and constructed genomic clone contigs around the Hd6 region.
Linkage analysis with P1-derived artificial chromosome clone-derived DNA markers
delimited Hd6 to a 26.4-kb genomic region. We identified a gene encoding the
alpha subunit of protein kinase CK2 (CK2 alpha) in this region. The Nipponbare
allele of CK2 alpha contains a premature stop codon, and the resulting truncated
product is undoubtedly nonfunctional. Genetic complementation analysis revealed
that the Kasalath allele of CK2 alpha increases days-to-heading. Map-based
cloning with advanced backcross progeny enabled us to identify a gene underlying
a quantitative trait locus even though it exhibited a relatively small effect on
the phenotype.