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E.g., Wessler, regeneration, PubMed ID 17578919.

expand all sections collapse all sections  Reference "Genetic manipulation and quantitative-trait loci mapping for nitrogen recycling in rice"
Reference ID 6598
Title Genetic manipulation and quantitative-trait loci mapping for nitrogen recycling in rice
Source Journal of experimental botany, 2002, vol. 53, pp. 917-925
Authors (6)
Abstract Immunocytological studies in this laboratory have suggested that NADH-dependent
glutamate synthase (NADH-GOGAT; EC 1.4.1.14) in developing organs of rice (Oryza
sativa L. cv. Sasanishiki) is involved in the utilization of glutamine
remobilized from senescing organs through the phloem. Because most of the indica
cultivars contained less NADH-GOGAT in their sink organs than japonica
cultivars, over-expression of NADH-GOGAT gene from japonica rice was
investigated using Kasalath, an indica cultivar. Several T0 transgenic Kasalath
lines over-producing NADH-GOGAT under the control of a NADH-GOGAT promoter of
Sasanishiki, a japonica rice, showed an increase in grain weight (80% as a
maximum), indicating that NADH-GOGAT is indeed a key step for nitrogen
utilization and grain filling in rice. A genetic approach using 98 backcross-
inbred lines (BC(1)F(6)) developed between Nipponbare (a japonica rice) and
Kasalath were employed to detect putative quantitative trait loci (QTLs)
associated with the contents of cytosolic glutamine synthetase (GS1; EC
6.3.1.2), which is probably involved in the export of nitrogen from senescing
organs and those of NADH-GOGAT. Immunoblotting analyses showed transgressive
segregations toward lower or greater contents of these enzyme proteins in these
BC(1)F(6). Seven chromosomal QTL regions were detected for GS1 protein content
and six for NADH-GOGAT. Some of these QTLs were located in QTL regions for
various biochemical and agronomic traits affected by nitrogen recycling. The
relationships between the genetic variability of complex agronomic traits and
traits for these two enzymes are discussed.

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