Reference "Linking differential domain functions of the GS3 protein to natural variation of grain size in rice"

Reference ID

54924

Title

Linking differential domain functions of the GS3 protein to natural variation of grain size in rice

Source

Proc Natl Acad Sci U S A, 2010, vol. 107, pp. 19579-19584

Authors (8)

Mao-H	Sun-S
Yao-J	Wang-C
Yu-S	Xu-C
Li-X	Zhang-Q

Abstract

Grain yield in many cereal crops is largely determined by grain size. Here we
report the genetic and molecular characterization of GS3, a major quantitative
trait locus for grain size. It functions as a negative regulator of grain size
and organ size. The wild-type isoform is composed of four putative domains: a
plant-specific organ size regulation (OSR) domain in the N terminus, a
transmembrane domain, a tumor necrosis factor receptor/nerve growth factor
receptor (TNFR/NGFR) family cysteine-rich domain, and a von Willebrand factor
type C (VWFC) in the C terminus. These domains function differentially in grain
size regulation. The OSR domain is both necessary and sufficient for
functioning as a negative regulator. The wild-type allele corresponds to medium
grain. Loss of function of OSR results in long grain. The C-terminal TNFR/NGFR
and VWFC domains show an inhibitory effect on the OSR function; loss-of-
function mutations of these domains produced very short grain. This study
linked the functional domains of the GS3 protein to natural variation of grain
size in rice.