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E.g., Wessler, regeneration, PubMed ID 17578919.

expand all sections collapse all sections  Reference "Natural Variation at the Pi-ta Rice Blast Resistance Locus"
Reference ID 54916
Title Natural Variation at the Pi-ta Rice Blast Resistance Locus
Source Phytopathology, 2003, vol. 93, pp. 1452-1459
Authors (4)
Abstract ABSTRACT The resistance gene Pi-ta protects rice crops against the fungal
pathogen Magnaporthe grisea expressing the avirulence gene AVR-Pita in a gene-for-
gene manner. Pi-ta, originally introgressed into japonica rice from indica
origin, was previously isolated by positional cloning. In this study, we report
the nucleotide sequence of a 5,113-base pair region containing a japonica
susceptibility pi-ta allele, which has overall 99.6% nucleotide identity to the
indica Pi-ta allele conferring resistance. The intron region shows the levels of
sequence diversity that typically differentiate genes from indica and japonica
rices, but the other gene regions show less diversity. Sequences of the Pi-ta
allele from resistant cultivars Katy and Drew from the southern United States
are identical to the resistance Pi-ta sequence. Sequences from susceptible
cultivars El Paso 144 and Cica 9 from Latin America define a third
susceptibility haplotype. This brings the total number of Pi-ta haplotypes
identified to four, including the resistance allele and three susceptibility
alleles. The Pi-ta locus shows low levels of DNA polymorphism compared with
other analyzed R genes. Understanding the natural diversity at the Pi-ta locus
is important for designing specific markers for incorporation of this R gene
into rice-breeding programs.

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