Reference "Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS"

Reference ID

5127

Title

Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS

Source

The Plant cell, 2000, vol. 12, pp. 2473-2484

Authors (11)

Yano-M	Katayose-Y	Ashikari-M	Yamanouchi-U
Monna-L	Fuse-T	Baba-T	Yamamoto-K
Umehara-Y	Nagamura-Y	Sasaki-T

Abstract

A major quantitative trait locus (QTL) controlling response to photoperiod, Hd1,
was identified by means of a map-based cloning strategy. High-resolution mapping
using 1505 segregants enabled us to define a genomic region of approximately 12
kb as a candidate for Hd1. Further analysis revealed that the Hd1 QTL
corresponds to a gene that is a homolog of CONSTANS in Arabidopsis. Sequencing
analysis revealed a 43-bp deletion in the first exon of the photoperiod
sensitivity 1 (se1) mutant HS66 and a 433-bp insertion in the intron in mutant
HS110. Se1 is allelic to the Hd1 QTL, as determined by analysis of two se1
mutants, HS66 and HS110. Genetic complementation analysis proved the function of
the candidate gene. The amount of Hd1 mRNA was not greatly affected by a change
in length of the photoperiod. We suggest that Hd1 functions in the promotion of
heading under short-day conditions and in inhibition under long-day conditions.