grain_icon  Literature Home |  Rice Genetics Newsletters |  Tutorial |  FAQ
E.g., Wessler, regeneration, PubMed ID 17578919.

expand all sections collapse all sections  Reference "DNA polymorphism in the blast disease resistance gene Pita of the wild rice Oryza rufipogon and its related species"
Reference ID 48837
Title DNA polymorphism in the blast disease resistance gene Pita of the wild rice Oryza rufipogon and its related species
Source Genes & genetic systems, 2009, vol. 84, pp. 121-136
Authors (2)
Abstract Intra-and interspecific DNA variations in the blast resistance gene Pita in wild
rice (Oryza rufipogon), cultivated rice (O. sativa), and two other related wild
rice species (O. meridionalis and O. officinalis) were analyzed to elucidate the
nucleotide polymorphism maintenance mechanisms and evolution of Pita in these
species. Nucleotide diversity at silent sites of O. rufipogon Pita was 0.0101,
an intermediate value relative to other O. rufipogon nuclear genes. A dimorphic
pattern of nucleotide polymorphism was detected in the O. rufipogon Pita region.
Inoculation of the blast fungus Magnaporthe oryzae verified that the O.
rufipogon Pita gene resides in a dimorphic sequence type. The resistance Pita
allele had lower levels of variation than the susceptibility pita allele. A
hypothesis of evolutionary relationships indicated that the amino acid mutation
in the O. rufipogon Pita protein responsible for the difference between
resistance and susceptibility occurred relatively recently. These results
suggested that the resistance Pita originated from the susceptibility pita.
Nucleotide diversity at replacement sites of the leucine-rich domain (LRD) of
both the resistance and susceptibility O. rufipogon pita was low. In tests of
neutrality, significantly negative values were detected for the LRD of O.
rufipogon susceptibility pita. The low nucleotide diversity at replacement sites
of the LRD of the susceptibility pita could be explained by purifying selection.
Comparison of Pita between O. rufipogon and O. officinalis revealed an excess of
nonconservative amino acid substitutions in the LRD, which could be related to
the host-pathogen interaction.

toggle section  Database Cross-References (1)
box  Proteins (0)
box  Markers (0)
box  QTL (0)
box  Genes (0)
toggle section  Ontologies (2)
box  Map Sets (0)
toggle section  Diversity Experiments (1)

Please note:
To request reprints, please contact the authors or the source/journal website. Due to copyright issues Gramene does not distribute reprints.